Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), PE-eFluor 610, eBioscience
Flow cytometry용 Mouse IgG2a kappa 동형 대조 항체로, PE-eFluor 610 형광체 결합 제품입니다. 실험 항체와 동일 농도로 사용하며, 488–561 nm 레이저에 의해 여기되고 607 nm에서 방출됩니다. 광민감성이 있어 어둡게 보관해야 합니다.
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Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), PE-eFluor 610
Applications
- Flow Cytometry (Assay-Dependent)
- Control (Assay-Dependent)
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBM2a |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637331 |
Additional Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 Series
- Brilliant Violet 421–786 Series
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780 Series
- Custom conjugation available
Product Specific Information
This monoclonal mouse IgG2a antibody serves as an isotype control for mouse IgG2a antibodies.
Reported application: Flow cytometric analysis.
Tested application: Flow cytometric analysis.
Use isotype control at the same concentration as the experimental antibody.
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®). Ensure instrument compatibility.
Light sensitivity: Sensitive to photo-induced oxidation; protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.
Excitation: 488–561 nm
Emission: 607 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgG2a is a subtype of immunoglobulin G produced by B cells in response to antigens. It is the second most abundant IgG subtype in serum and plays a key role in adaptive immune responses, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It also regulates immune responses such as T cell activation and B cell inhibition.
Mouse IgG2a is widely used in research and diagnostic applications (ELISA, Western blot, immunohistochemistry) and in therapeutic research (immunotherapy, vaccine development).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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