Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610, eBioscience

Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610

Applications

• Flow Cytometry (Flow): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor Series (450–660)
• FITC, PE, PE-Cyanine Series, PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Pacific Orange
• Functional Grade
• Custom conjugation available

Product Specific Information

The monoclonal mouse IgG1 κ immunoglobulin is useful as an isotype control.
This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis and tested on normal human peripheral blood cells.
Use the isotype control at the same concentration as the experimental antibody.

Fluorochrome characteristics:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm.
Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
Ensure instrument compatibility.

Light sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation guidance:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.

Excitation/Emission:
488–561 nm / 607 nm
Laser compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a key role in adaptive immune response.
Composed of two heavy and two light chains, it functions in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
Widely used in research applications including ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research such as immunotherapy and vaccine development.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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