Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610, eBioscience
PE-eFluor 610으로 결합된 Mouse IgG1 kappa 동형 대조 항체로 유세포 분석용으로 최적화됨. P3.6.2.8.1 클론 기반의 단일클론 항체. 488–561 nm 레이저에서 여기되고 607 nm에서 방출. 광산화에 민감하며 암조건에서 보관 권장.
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Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610
Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637444 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor Series (450–660)
- FITC, PE, PE-Cyanine Series, PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright Series (436–780)
- Pacific Orange
- Functional Grade
- Custom conjugation available
Product Specific Information
The monoclonal mouse IgG1 κ immunoglobulin is useful as an isotype control.
This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis and tested on normal human peripheral blood cells.
Use the isotype control at the same concentration as the experimental antibody.
Fluorochrome characteristics:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm.
Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®).
Ensure instrument compatibility.
Light sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation guidance:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.
Excitation/Emission:
488–561 nm / 607 nm
Laser compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigens. It is the most abundant IgG subtype in serum and plays a key role in adaptive immune response.
Composed of two heavy and two light chains, it functions in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
Widely used in research applications including ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research such as immunotherapy and vaccine development.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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