Thermo Fisher Scientific IL1F9 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 mg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Animal-Free Recombinant Human IL-36gamma (IL-1F9) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL1F9,uniProtId:Q9NZH8-1,ncbiNodeId:9606,antigenRange:18-169,antigenLength:169,antigenImageFileName:500-P316BT-25UG_IL1F9_Q9NZH8-1_Rabbit.svg,antigenImageFileNamePDP:500-P316BT-25UG_IL1F9_Q9NZH8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930357

Product Specific Information

AA Sequence of recombinant protein: SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-36gamma (IL-1F9). Anti-Human IL-36gamma (IL-1F9)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human IL-36gamma by sandwich ELISA (using 100 mL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human IL-36gamma (500-P316) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-36gamma.

Western Blot: To detect Human IL-36gamma by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human IL-36gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P316BT-1MG will be provided as 2 x 500 µg

Target Information

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.