Thermo Fisher Scientific H3K36me3 Recombinant Rabbit Monoclonal Antibody (RM491), ChIP-Verified
Histone H3의 Lys36me3에 특이적인 recombinant rabbit monoclonal antibody로, ChIP 검증 완료. Western blot, ICC, ELISA, ChIP, ChIP-Seq 등 다양한 응용에 사용 가능. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1–5 µg |
| ChIP-sequencing (ChIP-Seq) | 3 µg |
| Luminex (LUM) | 0.1–0.5 µg/mL |
| Peptide Array (Array) | 0.25 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM155 |
| Immunogen | Tri-methyl-peptide corresponding to tri-methyl-Histone H3 (Lys36) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2866505 |
Product Specific Information
This antibody reacts specifically to Histone H3 trimethylated at Lysine 36 (H3K36me3).
No cross-reactivity with monomethylated (H3K36me1) or dimethylated (H3K36me2) forms, or other methylations on histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone antibody DNA sequences from immunoreactive rabbits, and the best clones are selected for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Click here for Master Lot linking information (PDF)
Target Information
Histone H3 is a DNA-binding protein component of chromatin in all eukaryotic cells. Together with four core histones, it forms nucleosomes that compact and organize DNA.
Histones are key regulators of transcription, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes multiple post-translational modifications—such as methylation, acetylation, and phosphorylation—that modulate chromatin structure and transcription factor binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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