Thermo Fisher Scientific Phospho-CDC27 (Ser426) Polyclonal Antibody

Thermo Fisher Scientific Phospho-CDC27 (Ser426) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot and freeze at -20°C or below for long-term storage.
• Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid; dilute only before immediate use.

This affinity purified antibody specifically recognizes the phosphorylated form of human CDC27 at residue pS426. It shows minimal reactivity with non-phosphorylated CDC27 and does not cross-react with CDC27 phosphorylated at other sites.
BLAST analysis indicates reactivity with human, rat, dog, chicken, and chimpanzee CDC27 proteins (100% homology). Possible cross-reactivity with mouse CDC27 (89% homology). Cross-reactivity with other species has not been determined.

Target Information

The CDC27 protein shares strong similarity with yeast proteins Cdc27 (Saccharomyces cerevisiae) and nuc2 (Schizosaccharomyces pombe). It is a component of the Anaphase-Promoting Complex (APC), a conserved multi-subunit complex that catalyzes cyclin B-ubiquitin conjugate formation, leading to ubiquitin-mediated proteolysis of B-type cyclins.
CDC27 and other APC components contain TPR (tetratricopeptide repeat) domains, essential for protein-protein interactions. CDC27 interacts with mitotic checkpoint proteins including Mad2, p55CDC, and BUBR1, contributing to mitosis timing regulation.
Alternative splicing generates multiple isoforms of this gene.

Usage Notice

For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.