Thermo Fisher Scientific UBC12 Polyclonal Antibody
UBC12 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 ELISA에 적합. 사람, 생쥐, 랫드 등 다양한 종에 반응. Affinity chromatography로 정제된 액상 항체이며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA (ELISA) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Chimpanzee, Human, Mouse, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole goat serum immunized with a synthetic peptide corresponding to a C-terminal region near aa 150–183 of Human UBC12 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human UBC12 protein. 100% homology is observed for this protein from human, chimpanzee, frog, mouse, rat, and dog according to BLAST analysis. Reactivity with UBC12 proteins from other species is not known.
Target Information
The modification of proteins with ubiquitin is an essential cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves at least three classes of enzymes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins such as Cdc53/cullin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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