Thermo Fisher Scientific Slit1 Polyclonal Antibody
Thermo Fisher Scientific의 Slit1 Polyclonal Antibody는 마우스 및 랫트 SLIT-1 단백질을 인식하는 친화 정제 항체입니다. Western blot, ELISA, IP에 사용 가능하며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 485–515 of mouse SLIT-1 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse SLIT-1 protein.
A BLAST analysis suggests reactivity with mouse and rat SLIT-1 based on 100% homology for the immunogen sequence.
Cross-reactivity is expected with human, chicken, and frog SLIT-1 (94% positive by BLAST).
Cross-reactivity with other homologues has not been determined.
Target Information
SLIT1 is thought to act as a molecular guidance cue in cellular migration, mediated by interaction with roundabout homolog receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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