Thermo Fisher Scientific POLR2A Recombinant Rabbit Monoclonal Antibody (8H4A2)
POLR2A 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot 및 ELISA에 적합하며, 인간과 마우스 시료 반응. 고순도 Affinity Chromatography 정제, 액상 형태로 안정적인 보관 가능.
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Thermo Fisher Scientific POLR2A Recombinant Rabbit Monoclonal Antibody (8H4A2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H4A2 |
| Immunogen | Recombinant fusion protein containing amino acids 1135–1311 of human POLR2A (NP_000928.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911895 |
Product Specific Information
- Positive test controls: HeLa, Mouse brain
- Target localization: Nucleus
Immunogen sequence:
KTPSLTVFLL GQSARDAERA KDILCRLEHT TLRKVTANTA IYYDPNPQST VVAEDQEWVN VYYEMPDFDV ARISPWLLRV ELDRKHMTDR KLTMEQIAEK INAGFGDDLN CIFNDDNAEK LVLRIRIMNS DENKMQEEEE VVDKMDDDVF LRCIESNMLT DMTLQGIEQI SKVYMHL
Target Information
DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes transcription of DNA into RNA using ribonucleoside triphosphates as substrates. POLR2A is the largest subunit and a catalytic component of RNA polymerase II, synthesizing mRNA precursors and non-coding RNAs. It forms the polymerase active center with the second largest subunit and is central to the basal transcription machinery.
POLR2A includes mobile structural elements such as the clamp and jaws that interact with the DNA template. The bridging helix from RPB1 promotes translocation of Pol II by conformational changes during nucleotide addition. Transcription elongation is regulated by the phosphorylation of the CTD of RPB1, serving as a scaffold for factors controlling initiation, elongation, termination, and mRNA processing. Gene expression regulation depends on the balance of CTD-lysine methylation and acetylation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
(이미지 파일: MA5-42754_POLR2A_P24928-1_Rabbit.svg, MA5-42754_POLR2A_P24928-1_Rabbit_PDP.jpeg)
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