Thermo Fisher Scientific H3K36me3 Recombinant Rabbit Monoclonal Antibody (7W5T2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

ChIP assay (ChIP)

5 µg antibody for 5 µg-10 µg of Chromatin

-

ChIP-sequencing (ChIP-Seq)

1:50-1:100

-

Dot blot (DB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

7W5T2

Immunogen

A synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K36me3,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:36,antigenLength:136,antigenImageFileName:MA5-42755_H3K36me3_P68431-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42755_H3K36me3_P68431-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:H3K36me3,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:36,antigenLength:136,antigenImageFileName:MA5-42755_H3K36me3_Q71DI3-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42755_H3K36me3_Q71DI3-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2911896

Product Specific Information

Positive test controls include: HeLa, NIH/3T3, C6.

Immunogen sequence: MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.