Thermo Fisher Scientific H3K36me3 Recombinant Rabbit Monoclonal Antibody (7W5T2)
H3K36me3에 특이적인 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA, IP, ChIP 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성. HEK293 발현, 액상 형태, 고순도 친화 크로마토그래피 정제. 연구용 전용.
- 카탈로그번호
- MA542755
- 판매단위
- pk
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Thermo Fisher Scientific H3K36me3 Recombinant Rabbit Monoclonal Antibody (7W5T2)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg Chromatin |
| ChIP-sequencing (ChIP-Seq) | 1:50–1:100 |
| Dot blot (DB) | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7W5T2 |
| Immunogen | Synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911896 |
Product Specific Information
- Positive test controls: HeLa, NIH/3T3, C6
- Immunogen sequence:
MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with other core histones, binds to DNA forming the nucleosome structure. Histones play a crucial role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, alter chromatin structure and influence gene expression by enabling transcription factor binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: MA5-42755_H3K36me3_P68431-1_Rabbit.svg, MA5-42755_H3K36me3_Q71DI3-1_Rabbit.svg 등)
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