Thermo Fisher Scientific ACSL4 Recombinant Rabbit Monoclonal Antibody (4W3T9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000 - 1:20,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:800

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4W3T9

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_0752661) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ACSL4,uniProtId:O60488-1,ncbiNodeId:9606,antigenRange:42-321,antigenLength:711,antigenImageFileName:MA5-42756_ACSL4_O60488-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42756_ACSL4_O60488-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2911897

Product Specific Information

Positive test controls include: 293T, HepG2, Mouse liver, Rat liver. The target is usually found in the following locations: Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein.

Immunogen sequence: MAKRIKAKPT SDKPGSPYRS VTHFDSLAVI DIPGADTLDK LFDHAVSKFG KKDSLGTREI LSEENEMQPN GKVFKKLILG NYKWMNYLEV NRRVNNFGSG LTALGLKPKN TIAIFCETRA EWMIAAQTCF KYNFPLVTLY ATLGKEAVVH GLNESEASYL ITSVELLESK LKTALLDISC VKHIIYVDNK AINKAEYPEG FEIHSMQSVE ELGSNPENLG IPPSRPTPSD MAIVMYTSGS TGRPKGVMMH HSNLIAGMTG QCERIPGLGP KDTYIGYLPL

Target Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.