Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), APC, eBioscience

Applications

• Flow Cytometry (Flow): Tested up to 0.125 µg/test
• Publications: View 13 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 450
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15–19 kDa cytokine secreted by Th2 cells.

• Applications Reported: Used as a capture antibody for human IL-4 ELISA and for intracellular staining in flow cytometric analysis.
• Applications Tested: Tested in intracellular staining and flow cytometry of stimulated human peripheral blood cells. Recommended ≤0.125 µg/test (100 µL final volume). Optimal titration required.
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor. IL-4 and IL-13 share overlapping functions through receptor binding. STAT6 mediates IL-4 signaling. The IL-4 gene is located on chromosome 5q within a cytokine gene cluster (IL-3, IL-5, IL-13, CSF2). IL-4, IL-13, and IL-5 are co-regulated by long-range elements over a 120 kb region. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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