Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), APC, eBioscience
쥐 IL-4에 특이적인 단클론 항체로, APC 형광표지되어 Flow Cytometry에 최적화됨. 11B11 클론으로 중화능 보유. 0.25 µg/test 권장. 4°C 암소 보관, 동결 금지. 연구용으로 B세포 분화 및 면역반응 연구에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 67 publications |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11B11 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469493 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Brilliant UV 615
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 786
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine. This antibody is neutralizing and recognizes IL-4, also known as BCDF, BCGF-1, and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. It promotes proliferation and differentiation of B cells and, together with CD40 signaling, induces Ig isotype switching and upregulation of MHC class II and CD23.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on Th2-polarized and stimulated mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimal titration recommended.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, acting as a ligand for the IL-4 receptor. IL-4 has broad biological functions and shares overlapping roles with IL-13. STAT6 mediates IL-4 signaling. The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q. IL-4 and IL-13 are closely located and co-regulated by long-range elements over a 120 kb region. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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