Thermo Fisher Scientific Phospho-CDC25A (Thr507) Polyclonal Antibody
CDC25A 단백질의 Thr507 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 고순도 정제, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC25A (Accession P30304), corresponding to amino acid residues around phosphorylated Thr507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818302 |
Product Specific Information
This antibody detects endogenous levels of CDC25A only when phosphorylated at Threonine 507.
Target Information
CDC25A is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C, each functioning at different phases of the cell cycle.
CDC25A is required for progression through G1 and S phases and activates the cyclin-dependent kinase CDC2 by removing two phosphate groups.
CDC25A is specifically degraded in response to DNA damage, preventing cells with chromosomal abnormalities from dividing.
CDC25A is considered an oncogene, although its exact role in oncogenesis has not been fully demonstrated.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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