Thermo Fisher Scientific Human IL-12 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

Human IL-12 recombinant protein is a heterodimer composed of disulfide-linked p35 and p40 subunits

Molecular weight

75 kDa

Class

Recombinant

Type

Protein

Purity

≥95.5% by SDS-PAGE and HPLC

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 ≤ 1.0 ng/mL; determined by the dose-dependent IFN-gamma production of NK cells stimulated with IL-18.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Carrier-Free

Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in phosphate buffered saline, pH 7.2-pH 7.4, to a concentration of ≥0.2 mg/mL. Further dilutions should be made in sterile medium or buffered solution with a carrier protein.

Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at ≤-20°C. Avoid repeated freeze/thaw cycles.

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system`s response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.