Thermo Fisher Scientific H3K9me1 Polyclonal Antibody
H3K9me1에 특이적인 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합. Human과 Mouse 시료에 반응하며, 고순도 Affinity chromatography로 정제됨. PBS 및 50% glycerol buffer에 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HIST3H3 (Accession Q16695), corresponding to amino acid residues M1–L21 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899318 |
Product Specific Information
Antibody detects endogenous levels of Mono-Methyl-Histone H3 only when methylated at Lys9.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones directly change chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 can undergo various modifications, including methylation or acetylation of lysine and arginine residues, and phosphorylation of serine or threonine, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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