Thermo Fisher Scientific NEDD9 Monoclonal Antibody (OTI6E3), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

HEF-1 (also known as NEDD9) is a multifunctional protein involved in integrin-based signaling pathways influencing cell motility, growth, apoptosis, and oncogenic transformation.
It belongs to the Cas family of docking proteins, which are substrates of focal adhesion kinase (FAK) and Src family tyrosine kinases—both key targets in drug development.
HEF1 overexpression promotes transcription of genes associated with motility and invasiveness, including metalloproteinases, MLCK, p160ROCK, and ErbB1.
It also mediates apoptosis in epithelial-derived cell lines such as MCF7 and HeLa.
Overexpression of a related protein, BCAR1 (p130Cas), has been linked to tamoxifen resistance, underscoring the significance of this protein family in cancer prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.