Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (TU-01)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 20 publications 20 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

1-4 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Many, Mouse, Pig

Published species

Bovine, Deer, Human, Mouse, Non-human primate, Pig, Protozoa, Rat, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TU-01

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Tubulin,uniProtId:Q71U36-1,ncbiNodeId:9606,antigenRange:1-451,antigenLength:451,antigenImageFileName:MA1-19162_alpha_Tubulin_Q71U36-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19162_alpha_Tubulin_Q71U36-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070259

Product Specific Information

This antibody recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.

HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins).

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Western Blot: Reducing conditions.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.