
Thermo Fisher Scientific NF-H Monoclonal Antibody (NF-01)
NF-H 단백질의 인산화된 에피토프를 인식하는 단클론 항체로, 신경세포 표지에 유용합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, Protein A로 정제된 액상 항체입니다. 4°C에서 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat, Human, Mammal, Mouse, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NF-01 |
| Immunogen | Pellet of pig brain cold-stable proteins after depolymerization of microtubules |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077145 |
Product Specific Information
This antibody recognizes a phosphorylated epitope on the heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are valuable neuronal cell type markers because these proteins are abundant in the nervous system, expressed only in neurons, and biochemically very stable.
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed mainly of three major proteins: NF-L, NF-M, and NF-H. The expression of these neuronal intermediate filament proteins is tissue-specific and developmentally regulated. NF-H plays a key role in maintaining neuronal caliber and is implicated in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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