Thermo Fisher Scientific PROK1 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific PROK1 Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography employing an immobilized Human EG-VEGF matrix.

Sandwich ELISA:
To detect Human EG-VEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human EG-VEGF (500-P188GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human EG-VEGF.

Western Blot:
To detect Human EG-VEGF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human EG-VEGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and restricted to steroidogenic glands. Expression is often complementary to VEGF, suggesting coordinated function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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