Thermo Fisher Scientific PROK1 Polyclonal Antibody, PeproTech
Human EG-VEGF(PROK1)를 인식하는 염소 유래 폴리클로날 항체로, Western blot과 ELISA에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로만 사용 가능하며 -20°C에서 보관.
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Thermo Fisher Scientific PROK1 Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human EG-VEGF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929726 |
Product Specific Information
AA Sequence of recombinant protein:
AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography employing an immobilized Human EG-VEGF matrix.
Sandwich ELISA:
To detect Human EG-VEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human EG-VEGF (500-P188GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human EG-VEGF.
Western Blot:
To detect Human EG-VEGF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human EG-VEGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and restricted to steroidogenic glands. Expression is often complementary to VEGF, suggesting coordinated function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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