Thermo Fisher Scientific NNT1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3.
Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:

• To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody.
• This biotinylated polyclonal antibody, combined with PeproTech Polyclonal Anti-Human NNT-1/BCSF-3 (500-P186) as a capture antibody, detects at least 0.2–0.4 ng/well of Recombinant hNNT-1/BCSF-3.

Western Blot:

• To detect Human NNT-1/BCSF-3 by Western Blot, use 0.1–0.2 µg/mL.
• In conjunction with compatible secondary reagents, the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P186BT-1MG is provided as 2 × 500 µg.

Target Information

NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in lymph nodes and spleen, it activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing tyrosine phosphorylation of these receptors. In vitro, it supports survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.