Thermo Fisher Scientific PROK1 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hEG-VEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EG-VEGF (500-P188G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEG-VEGF.

Western Blot: To detect Human EG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P188GBT-1MG will be provided as 2 x 500 µg

Target Information

Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands. Its expression is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.