Thermo Fisher Scientific PROK1 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific PROK1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hEG-VEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EG-VEGF (500-P188G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hEG-VEGF.

Western Blot:
To detect Human EG-VEGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human EG-VEGF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P188GBT-1MG will be provided as 2 × 500 µg.

Target Information

Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to steroidogenic glands. Expression is often complementary to VEGF, suggesting coordinated function.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.