Thermo Fisher Scientific Rhodamine Monoclonal Antibody (11H10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

ELISA (ELISA)

1:10,000-1:30,000

-

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

11H10

Immunogen

Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.

Target Information

Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.