Thermo Fisher Scientific HSP27 Monoclonal Antibody (G3.1)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1:10,000–1:50,000

Immunoprecipitation (IP)

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control.
Cross-reactivity with HSP27 from other mammalian sources is likely.
No cross-reactivity occurs with HSP70, HSP90, or HSP104.

Target Information

In response to environmental stress, cells increase expression of heat shock proteins.
HSPB1 (heat shock protein beta-1 or HSP27) is a small molecular chaperone that maintains denatured proteins in a folding-competent state.
It contributes to stress resistance and actin organization, and regulates phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene can result in Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy type 2B.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.