Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), APC, eBioscience

Applications

Product Specifications

Additional Formats

• Unconjugated
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 605 / 650 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. It is expressed mainly on activated T and B lymphocytes. PD-L1 (B7-H1) and PD-L2 (B7-DC) are known ligands for PD-1, showing overlapping functions with constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.

J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes, similar to PD-1-deficient cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes. Recommended ≤0.5 µg/test (in 100 µL final volume).
Excitation/Emission: 633–647 nm / 660 nm (Red Laser)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-cell activation is self-limited by inhibitory receptors such as PD-1 (PDCD-1), which binds ligands PD-L1 and PD-L2 on antigen-presenting cells. Upon ligand binding, PD-1 transmits inhibitory signals that suppress immune activation in the absence of danger signals. PD-1 deficiency leads to hyperactivated immune responses and autoimmune diseases. Although its predicted molecular weight is 55 kDa, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures.

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