Thermo Fisher Scientific NEFM Monoclonal Antibody (OTI1A4), TrueMAB

Thermo Fisher Scientific NEFM Monoclonal Antibody (OTI1A4), TrueMAB

Applications and Tested Dilutions

• Western Blot (WB): 1:1,000–1:4,000
• Immunocytochemistry (ICC/IF): 1:100

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final concentration of approximately 1 mg/mL.
• For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Neurofilaments (NFs) are intermediate filaments found almost exclusively in neurons, particularly in large axons. They consist of three subunits—NF-L, NF-M, and NF-H—that share a coiled-coil core domain but differ in terminal sequences. NF-M plays a critical role in axonal size and cytoskeletal architecture through its phosphorylated tail domain. Phosphorylation and O-GlcNAcylation regulate NF-M function and filament formation. Antibodies against neurofilament subunits serve as valuable neuronal markers due to their abundance and stability in the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.