Thermo Fisher Scientific Steroidogenic Factor 1 (SF-1) Monoclonal Antibody (NR5A1, 3397)
인간 SF-1 단백질을 인식하는 마우스 단일클론 항체로, IHC(P) 및 Peptide Array에 사용 가능. Protein A/G로 정제된 액상 형태이며, 4°C에서 보관. 스테로이드 생성 효소 발현 연구 및 내분비계 발달 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NR5A1, 3397 |
| Immunogen | Recombinant fragment (around aa220–461) of human SF-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- For formalin-fixed tissues: heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at room temperature for 20 min.
Target Information
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system.
It is essential for fetal development of the hypothalamus, pituitary gland, gonads, and adrenal gland.
SF-1 promotes transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family.
It contains two activation function domains (AF1 and AF2) necessary for interaction with other nuclear receptors and coactivators/corepressors such as SRC-1, GRIP1, CREB binding protein (CBP), and SMRT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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