Thermo Fisher Scientific Phospho-p38 MAPK (Thr180, Tyr182) Recombinant Superclonal Antibody (B10HCLC)
인간 p38 MAPK의 Thr180/Tyr182 인산화 부위를 인식하는 재조합 Superclonal 항체. WB, IHC, ChIP 등 다양한 응용 가능. 폴리클로날의 민감도와 모노클로날의 특이성을 결합한 고재현성 항체. 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
| ChIP assay (ChIP) | 1 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | B10HCLC |
| Immunogen | Phosphopeptide corresponding to amino acids 176–186 of human p38 MAPK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532554 |
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They consist of multiple recombinant monoclonal antibodies, combining polyclonal sensitivity with monoclonal specificity, and ensuring lot-to-lot consistency. These antibodies recognize multiple epitope sites, providing high detection sensitivity for low-abundance targets. Each lot reproduces the same defined antibody population, eliminating variability typical of polyclonal antibodies.
Note: Formerly called “Recombinant polyclonal antibody”, now rebranded as “Recombinant Superclonal™ antibody”. Product and performance remain unchanged.
Target Information
MAPK14 (p38 alpha) is a stress-activated protein kinase regulating cellular responses to stress and inflammation. It is activated by dual phosphorylation at Thr180 and Tyr182. Four isoforms exist with differing tissue expression and affinities. Upon activation by stimuli such as TNF or IL-1, MAPK kinase-3/6 phosphorylates p38 MAPK, which then activates transcription factors including ATF-2, CHOP-1, and MEF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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