Thermo Fisher Scientific Phospho-SHC (Tyr427) Polyclonal Antibody
Phospho-SHC (Tyr427) 폴리클로날 항체로 인간, 마우스, 랫트에 반응합니다. Western blot 및 IHC(파라핀)에서 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 버퍼에 0.02% sodium azide 함유, 1 mg/mL 농도. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Shc around the phosphorylation site of Tyrosine 427 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553683 |
Product Specific Information
This antibody detects endogenous protein at molecular weights of 46, 52, and 66 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex.
Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts.
It is required for the ubiquitination and degradation of p27 in mammalian cells.
In normal tissues, Skp2 is expressed in tonsil and placenta.
In tumor tissues, Skp2 shows wide expression, including colon, prostate, pancreas, skin, lung, breast, ovarian, and endometrial cancers.
Research suggests Skp2 plays a role in oncogenesis, particularly in lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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