Thermo Fisher Scientific VEGF Polyclonal Antibody, HRP

Thermo Fisher Scientific VEGF Polyclonal Antibody, HRP

Applications and Tested Dilution

• Western Blot (WB): 1:1,000–1:5,000
• Immunohistochemistry (IHC): 1:500–1:2,500

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles. Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid; dilute only before immediate use.
• Antibody heated to 56°C for 30 minutes for purification.
• Recognizes both native and recombinant human VEGF-165 in ELISA and other immunoassays.
• Recommended to use normal rabbit IgG as a control.

Target Information

VEGF (Vascular Endothelial Growth Factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein that plays a critical role in angiogenesis, promoting tumor progression and metastasis. It affects vascular endothelium by enhancing cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. These isoforms arise from alternative splicing of a single VEGF gene and interact with tyrosine kinase receptors VEGFR-1 (flt-1) and VEGFR-2 (flk-1/KDR), primarily expressed on endothelial cells.
VEGF and its receptors are fundamental regulators in embryonic vascular development. The soluble form sFLT1 binds VEGF with high affinity and inhibits angiogenesis. Elevated VEGF levels are associated with POEMS syndrome (Crow-Fukase syndrome), affecting multiple organs.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.