Thermo Scientific™

Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for자세히 알아보기

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade Sulfo-NHS:

• Sulfo-NHS—use with EDC crosslinker to prepare amine-reactive NHS ester reagents
• High quality—identity and purity confirmed by several tests, including quantitative NMR
• Product integrity—enhanced level of testing and characterization compared to standard grade
• Lot retention—ample supply of past lots retained to ensure future process testing
• Change management—Change Control Notification (CCN) service
• Consistent manufacture—batch-specific manufacturing documentation review

Applications:
• Improve efficiency of EDC coupling reactions
• Convert carboxyls to amine-reactive sulfo-NHS esters
• Membrane-impermable for cell surface labeling
• Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-NHS (N-hydroxysulfosuccinimide)
Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

사양

화학물질 반응성Amine-Carboxyl

CleavableNo

분자량217.13

페길레이티드No

세포 투과성No

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분Carbodiimide

스페이서Short (<10 Å)

형태Powder

수량500 mg

용해도Water

형식Standard, Single-use, Premium-grade

수용성Yes

제품 유형Crosslinker

Unit SizeEach