Thermo Scientific™

Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product자세히 알아보기

Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Features of Sulfo-SMCC:

• Sulfo-SMCC—a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
• High quality—identity and purity confirmed by several tests, including quantitative NMR
• Product integrity—enhanced level of testing and characterization compared to standard grade
• Lot retention—ample supply of past lots retained to ensure future process testing
• Change management—Change Control Notification (CCN) service
• Consistent manufacture—batch-specific manufacturing documentation review

Applications:
• Enzyme labelling of antibodies - both enzyme and antibody specificity can be preserved
• Create specific bioconjugates via one- or two-step crosslinking reactions
• Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

사양

화학물질 반응성Amine-Sulfhydryl

CleavableNo

분자량436.37

페길레이티드No

스페이서 암 길이8.3 Å

세포 투과성No

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분Maleimide, Sulfo-NHS Ester

스페이서Short (<10 Å)

형태Powder

수량100 mg

용해도Water

형식Standard, Single-use, Premium-grade

수용성Yes

제품 유형Crosslinker

Unit SizeEach