Thermo Scientific™

Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications자세히 알아보기

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade EDC:

• EDC—carbodiimide crosslinker for conjugation between carboxylates and amines
• High quality—identity and purity confirmed by several tests
• Product integrity—enhanced level of testing and characterization compared to standard grade
• Lot retention—ample supply of past lots retained to ensure future process testing
• Change management—Change Control Notification (CCN) service
• Consistent manufacture—batch-specific manufacturing documentation review

Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.

Specifications of Premium Grade EDC
We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
• Visual: White crystalline powder, free of foreign matter
• Identity: IR scan shows only peaks characteristic of EDC
• Water content: Karl Fischer titration, ≤ 2%
• Trace metals: All metals, including lead, ≤ 50 ppm

Applications:
• Conjugate carboxyl and amino groups among peptides and proteins
• Couple haptens to immunogenic carrier proteins (e.g., attach a peptide to KLH)
• Immobilize peptide antigens to affinity-purify antibodies
• Create NHS-activated, amine-reactive labeling compounds
• Crosslink proteins to carboxyl coated beads or surfaces
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

사양

화학물질 반응성Amine-Carboxyl

CleavableNo

분자량191.7

페길레이티드No

스페이서 암 길이0.0 Å

세포 투과성No

배송 조건Room Temperature

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분Carbodiimide

스페이서Short (<10 Å)

형태Powder

수량25 g

용해도Water

형식Standard, Single-use, Premium-grade

수용성Yes

제품 유형Crosslinker

Unit SizeEach