Thermo Fisher Scientific Vimentin Polyclonal Antibody
Vimentin 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 적합. 인간, 마우스, 랫 등 반응성 보유. 고순도 항원 친화 크로마토그래피 정제. 안정적인 PBS 기반 저장용액 구성으로 장기 보관 가능.
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Thermo Fisher Scientific Vimentin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:50,000 | View 11 publications |
| Immunohistochemistry (IHC) | – | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 8 publications |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | 1:100–1:500 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 61–426 of Human Vimentin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2544707 |
Product Specific Information
- Recommended positive controls: 293T, HeLa, MDA-MB-231, U87-MG, SK-N-SH, IMR32, SK-N-AS, NIH-3T3, A549
- Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (85%), Dog (98%), Chicken (92%), Chimpanzee (99%), Bovine (98%), Guinea pig (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport between the nucleus and plasma membrane. During development, vimentin is expressed with desmin and later replaced by tissue-specific IFPs. Vimentin contributes to steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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