Thermo Fisher Scientific TOMM20 Recombinant Rabbit Monoclonal Antibody (ST04-72)
TOMM20 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 높은 특이성과 일관된 로트 간 재현성 제공. 단백질 A로 정제된 액상 항체로 4°C 또는 -20°C 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:800 | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:1,000 | View 2 publications |
| Flow Cytometry (Flow) | 1:1,000 | - |
| in situ PLA (PLA) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST04-72 |
| Immunogen | Recombinant protein within Human TOMM20 aa 1–145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809438 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
The mitochondrial preprotein translocases of the outer membrane (Tom) complex mediates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane.
Tom20 and Tom22 form a receptor complex that initiates insertion of newly synthesized proteins into the outer membrane and directs precursor proteins into the general import pore (GIP).
In yeast, Tom22 acts as both a receptor and docking point for Tom20 and the GIP, making it essential for protein import into mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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