Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), APC, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (MIH4), APC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1 µg)/test
Publications: View 29 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 645
• Request custom conjugation

Product Specific Information

The MIH4 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. It is predominantly expressed on activated T and B lymphocytes.

PD-L1 (B7-H1) and PD-L2 (B7-DC) are PD-1 ligands, showing overlapping functions and expression on normal tissues and activated antigen-presenting cells.
The MIH4 antibody recognizes a different epitope than clone J105.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on PHA-stimulated human peripheral blood cells.
Use at 5 µL (1 µg) per test in a final volume of 100 µL (10⁵–10⁸ cells/test).

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

PD-1 (PDCD-1) is an inhibitory receptor expressed on activated T cells, regulating immune responses by binding to ligands PDL-1 and PDL-2 on antigen-presenting cells. Binding inhibits immune activation in the absence of danger signals (e.g., LPS). PD-1 knockout mice exhibit hyperactivated immune systems and autoimmune disease. Despite its predicted molecular weight, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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