Thermo Fisher Scientific CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), eFluor 450, eBioscience
HIS51 클론의 CD90.1 (Thy-1.1) 단클론 항체로, eFluor 450 형광체가 결합된 제품입니다. 쥐 및 마우스 CD90 분석에 적합하며, Flow Cytometry에 최적화되어 있습니다. 405/445 nm 파장에서 작동하며, 4°C 암소 보관이 권장됩니다.
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Thermo Fisher Scientific CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 52 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS51 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_1272257 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In rats, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, neurons, inflamed endothelia, and other cell types.
In CD90.1-expressing mouse strains (PL and AKR), CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes, and mature T cells.
Applications Reported:
This HIS51 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. It can be used at ≤0.25 µg per test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®. It emits at 445 nm and is excited with the Violet laser (405 nm). Ensure instrument compatibility.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer cells, and fibroblasts. Thy-1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other brain-related events.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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