Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), eFluor 450, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), eFluor 450

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the immunoglobulin superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometry on stimulated mouse splenocytes (≤ 0.25 µg/test)
• Recommended: Empirical titration for optimal performance
• Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells and various lymphoma cell lines. It provides regulatory signals for T lymphocytes through CD28 and CTLA4 binding.

Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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