Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), eFluor 450, eBioscience
Mouse CD80 (B7-1) 단백질을 인식하는 16-10A1 클론의 단클론 항체로, eFluor 450 형광체 결합. Flow Cytometry에 적합하며 0.25 µg/test로 최적화됨. Violet laser(405 nm)로 여기, 445 nm에서 방출. 면역세포 활성화 연구용.
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Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), eFluor 450
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 60 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Mouse, Pig |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16-10A1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574028 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the immunoglobulin superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometry on stimulated mouse splenocytes (≤ 0.25 µg/test)
- Recommended: Empirical titration for optimal performance
- Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells and various lymphoma cell lines. It provides regulatory signals for T lymphocytes through CD28 and CTLA4 binding.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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