Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte activation and development.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of 24-hour Con A-stimulated mouse splenocytes
• Recommended Usage: ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Fluorochrome: eFluor® 450 (alternative to Pacific Blue®)
• Excitation / Emission: 405 nm / 445 nm
• Laser: Violet Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It is expressed on activated platelets and lymphocytes, playing a role in signal transduction and proliferation. CD69 expression is induced upon T-cell activation and may also transmit signals in NK cells and platelets. Alternative splicing yields multiple transcript variants. Dysregulation is associated with diseases such as coccidioidomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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