Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), eFluor 450, eBioscience
마우스 CD86(B7-2) 표면 수용체를 인식하는 GL1 클론 단일클론 항체로, eFluor 450 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며 Violet laser(405 nm)로 여기됩니다. 면역반응 연구 및 T세포 활성화 분석에 적합합니다.
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Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 96 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GL1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574030 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 605
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The GL1 monoclonal antibody reacts with mouse CD86 (B7-2), an approximately 80 kDa surface receptor belonging to the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation through the BCR complex, CD40, and cytokine receptors. It serves as a counter-receptor for T cell surface molecules CD28 and CD152 (CTLA-4), playing a critical role in T-B cell interaction, T cell costimulation, and antibody production.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes, ≤0.25 µg/test (100 µL final volume).
Fluorophore Information: eFluor® 450 emits at 445 nm and is excited at 405 nm using the Violet laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD86 is a costimulatory molecule that interacts with CTLA4 and CD28 on T cells. It is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells. Binding of CD86 to CD28 activates T cells, while binding to CTLA-4 inhibits activation and modulates immune response. Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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