Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), FITC, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, APC, APC-eFluor 780, Biotin, Brilliant UV 496/563/615/661/737, Brilliant Violet 421/480/605/650/711/786, eFluor 450, Functional Grade, NovaFluor Blue/Red/Yellow series, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436/600/780.
Custom conjugation available upon request.

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.

Applications Reported: Flow cytometry and immunohistochemistry (frozen tissue).
Applications Tested:

• Flow cytometry: ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• IHC (Frozen): ≤10 µg/mL.
  Antibody should be titrated for optimal performance.

Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It comprises CD3 gamma, delta, epsilon, and zeta subunits, encoded by closely linked genes on human chromosome 11. These subunits contain cytoplasmic tails with tyrosine-based motifs that mediate signaling molecule interactions. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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