Thermo Fisher Scientific PTPRC Monoclonal Antibody (OTI1B6), TrueMAB
인간 PTPRC(CD45)을 인식하는 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 고순도 친화 크로마토그래피 정제 제품으로, 동결건조 상태로 제공되며 재구성 후 1 mg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B6 |
| Immunogen | Full length human recombinant protein of human PTPRC produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, constituting about 10% of lymphocyte surface proteins. It is absent in non-hematopoietic cells and tissues.
CD45 plays a crucial role in lymphocyte development and antigen signaling by regulating Src-family kinases. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains.
CD45RA, one isoform, shows subtype-specific expression among lymphoid cells. CD45 regulates T- and B-cell receptor signaling, modulates cytokine receptor pathways via JAK kinases, and influences cell survival, apoptosis, and immune functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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