Thermo Fisher Scientific AIRE Monoclonal Antibody (5H12), eBioscience

Thermo Fisher Scientific AIRE Monoclonal Antibody (5H12), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• Alexa Fluor 488
• eFluor 660
• Custom conjugation available upon request

Product Specific Information

This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE), a 58-kDa transcription factor highly expressed in mature medullary thymic epithelial cells (mTECs). Expression in peripheral tissues remains unclear, although AIRE mRNA is detected in lymph nodes, stromal cells, monocytes, and dendritic cells.

AIRE plays a key role in thymocyte development and regulation of tissue-specific antigen expression during negative selection. It is also involved in chemokine expression and antigen presentation.

The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.

Applications Reported: Flow cytometry, immunoprecipitation, western blotting, immunohistochemical staining (frozen tissue).

Applications Tested: Immunohistochemistry on frozen 5-week-old mouse thymus at ≤5 µg/mL. Optimal performance requires titration.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

AIRE is a transcriptional regulator that binds DNA as a dimer or tetramer and regulates gene expression. It contains an HSR domain, two PHD-type zinc fingers, and a SAND domain, forming nuclear complexes and interacting with CBP and CREBBP coactivators. Three isoforms are known, one potentially subject to nuclear-mediated decay (NMD).

AIRE mediates E3 ubiquitin ligase activity and is crucial for immune regulation. Mutations cause autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED), a rare autosomal-recessive disorder.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.