Thermo Fisher Scientific CD38 Monoclonal Antibody (90), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD38 Monoclonal Antibody (90), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Immunohistochemistry (IHC): Not tested
• Flow Cytometry (Flow): 0.5 µg/test
  • Tested on mouse splenocytes
  • Recommended titration for optimal performance
  • Define test as 100 µL final volume per sample (10⁵–10⁸ cells/test)

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395
• Brilliant Violet 480
• Brilliant Violet 605
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The 90 monoclonal antibody reacts with mouse CD38, a ~42 kDa type II transmembrane protein. CD38 expression increases during B-cell differentiation and is down-regulated in germinal center B cells and mature plasma cells. It is also found on subsets of thymocytes, mature T cells, and NK cells.
Crosslinking of CD38 induces B-cell proliferation, enhanced by IL-4 and LPS. CD38 acts as a counter-receptor for CD31 and functions as an ectoenzyme with cyclase and hydrolase activity, contributing to lymphocyte activation and differentiation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes
• Excitation/Emission: 633–647 nm / 780 nm (Red laser)
• Light Sensitivity: Protect from light (photo-induced oxidation)
• Fixation: Compatible with IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57); store up to 3 days at 4°C in the dark with minimal brightness loss.

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein that induces activation, proliferation, and differentiation of lymphocytes and mediates apoptosis of progenitor cells. It functions as a multi-catalytic ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a cell surface receptor.
Antibodies to CD38 are useful for lymphoma and leukemia subtyping, plasma cell detection (e.g., myeloma identification), and as markers for activated B and T cells. CD38 is expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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