Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), APC-eFluor 780, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.125 µg)/test
• View 16 Publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 480 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S / 690
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 660 / 755
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

Description:
The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 binds to CD80 (B7-1) and CD86 (B7-2) and acts as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported:

• Flow cytometric analysis.

Applications Tested:

• Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
• Recommended use: 5 µL (0.125 µg) per test.
• A test is defined as the amount of antibody staining a cell sample in 100 µL volume (10⁵–10⁸ cells/test).

Fluorochrome Characteristics:

• Excitation: 633–647 nm (Red Laser)
• Emission: 780 nm
• Light sensitivity: Protect from light (photo-induced oxidation sensitive)

Fixation:

• Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Filtration:

• 0.2 µm post-manufacturing filtered.

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell surface glycoprotein belonging to the immunoglobulin superfamily. It is expressed on most peripheral blood T lymphocytes and functions as a critical costimulatory receptor by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering anergic or apoptotic states. In mice, CD28 is expressed on all CD4⁺ and CD8⁺ T cells, with highest expression on immature thymocytes. Dysregulation of CD28 is associated with diseases such as mycosis fungoides and Sezary’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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