Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), PE-eFluor 610, eBioscience
마우스 CD11c를 표적하는 N418 단클론 항체로, PE-eFluor 610 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.25 µg/test 권장. 488–561 nm 레이저로 여기, 607 nm에서 방출. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) | - | View 3 publications |
| Flow Cytometry | 0.25 µg/test | View 122 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574529 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395–805)
- Brilliant Violet (421–711)
- eFluor 450, 506, 615, 660
- FITC
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright (436–780)
- NovaFluor (Blue, Yellow series)
- Request custom conjugation
Product Specific Information
Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alpha X). CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. It is expressed by dendritic cells, intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 binds to CD11c on splenic dendritic cells in T-dependent areas of the mouse spleen and precipitates a 150, 90 kDa heterodimer.
Applications Reported:
Flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using mouse splenocytes. Recommended ≤0.25 µg/test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Optimize titration for best performance.
Fluorophore Information:
PE-eFluor® 610: Excitation 488–561 nm, Emission 607 nm.
Recommended filter: 610/20 band pass (PE-Texas Red® equivalent).
Ensure instrument compatibility.
Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored up to 3 days at 4°C (dark) in:
- IC Fixation Buffer (Product #00-8222)
- 1-step Fix/Lyse Solution (Product #00-5333)
Clone-specific performance should be verified empirically.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD11, together with CD18, forms a heterodimeric adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
- CD11b: Found in monocytes, macrophages, NK cells, some B/T cells, granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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