Thermo Fisher Scientific EpCAM Monoclonal Antibody (OTI2C8), TrueMAB
EpCAM 단백질을 인식하는 Mouse IgM 단일클론 항체로, Western Blot 및 IHC(Paraffin)에 적합. Human 시료 반응성. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도. EpCAM 관련 세포 접착 및 종양 연구용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 24–265 of human EpCAM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve ~1 mg/mL antibody concentration.
- For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle regulators cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial-derived tumors.
Ep-CAM is localized on the basolateral surface of most simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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