Thermo Fisher Scientific Patched1 Monoclonal Antibody (OTI3c2), TrueMAB
Patched1 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 Immunocytochemistry에 적합. E.coli 발현 재조합 단백질(aa 122-436)을 면역원으로 제작. 동결건조 형태로 제공되며, 1 mg/mL 농도로 재구성 가능. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3c2 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 122–436 of human Patched1 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The Patched1 (PTCH1) gene encodes a receptor for the Sonic Hedgehog (SHH) signaling pathway, which plays a critical role in embryonic development and tumorigenesis.
This gene acts as a tumor suppressor, and mutations have been linked to basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinoma of the bladder, and holoprosencephaly.
Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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