Thermo Fisher Scientific NCOA3 Polyclonal Antibody
인간 NCOA3 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 종에서 교차 반응 가능. Western blot, IHC, ELISA, IP에 사용 가능. Affinity chromatography로 정제된 액상 항체로 장기 보관 시 -20°C 보관 권장.
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- 600-401-A40x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NCOA3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:175,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from rabbit serum immunized with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.24 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e, and f). A BLAST analysis suggests cross-reactivity with NCOA3 from human and macaque sources (100% homology with the immunizing sequence). Partial reactivity expected with NCOA3 from dog, bovine, mouse, and rat sources based on partial sequence homology. Cross-reactivity with other species has not been determined.
Target Information
AIB1-AC3 recognizes AIB1, a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160 kDa. The AIB1 gene is located at 20q12 and is known to be amplified in breast tumors and pancreatic cancer cell lines. AIB1 interacts in a ligand-dependent manner with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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