Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Red 710

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S / Red 725 / Yellow 660 / Yellow 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom Conjugation Available

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with a molecular weight of 50–80 kDa.
CD16 exists in two forms:

• CD16A (FcγRIIIA): Found in macrophages and NK cells (polypeptide-anchored)
• CD16B (FcγRIIIB): Found in neutrophils (GPI-anchored)

Two polymorphisms (NA-1 and NA-2) of CD16B are known, with NA-2 homozygotes exhibiting lower phagocytic capacity. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.2 µg)/test.

Note:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.

Recommended Usage:
Add 5 µL of CellBlox Plus Blocking Buffer per sample (10³–10⁸ cells/test). For single-color controls, use 5 µL per 100 µL cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures designed for optimized fluorescence performance and minimized spectral spillover.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

CD16 encodes a receptor that binds the Fc region of IgG and plays a role in immune complex clearance and antibody-dependent cytotoxicity.

• FCGR3A: Expressed on NK cells (transmembrane form)
• FCGR3B: Expressed on neutrophils (PI-anchored form)
  Mutations are associated with Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus.
  Alternative splicing produces multiple isoforms.

Usage Notice

For Research Use Only.
Not for diagnostic or therapeutic applications.
Not for resale without authorization.

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